helios cytof system Search Results


helios cytof instrument  (fluidigm)
93
fluidigm helios cytof instrument
Schematic of experimental design and data analyses. Plasma and sera from 27 individuals with Long COVID (LC) and 16 individuals without LC (Non-LC) were subjected to Olink and serological analyses. PBMCs from the same individuals were subjected to RNAseq analysis, as well as to <t>CyTOF</t> analysis at baseline, or following a 6-hour stimulation with peptides derived from SARS-CoV-2 spike proteins (see Methods) to analyze T cell responses. The cells for CyTOF were treated with viability marker, fixed, and stained with a 39-parameter panel prior to analysis on a <t>CyTOF</t> <t>instrument.</t> The indicated tools on the right were then used for analyses of the resulting high-dimensional datasets.
Helios Cytof Instrument, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+cytof+system/Helios/bio_rxiv__2023__02__09__527892-210-32-9
Average 93 stars, based on 1 article reviews
helios cytof instrument - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
helix cyto labeling kit red dye 1  (Dynamic Biosensors)
90
Dynamic Biosensors helix cyto labeling kit red dye 1
Schematic of experimental design and data analyses. Plasma and sera from 27 individuals with Long COVID (LC) and 16 individuals without LC (Non-LC) were subjected to Olink and serological analyses. PBMCs from the same individuals were subjected to RNAseq analysis, as well as to <t>CyTOF</t> analysis at baseline, or following a 6-hour stimulation with peptides derived from SARS-CoV-2 spike proteins (see Methods) to analyze T cell responses. The cells for CyTOF were treated with viability marker, fixed, and stained with a 39-parameter panel prior to analysis on a <t>CyTOF</t> <t>instrument.</t> The indicated tools on the right were then used for analyses of the resulting high-dimensional datasets.
Helix Cyto Labeling Kit Red Dye 1, supplied by Dynamic Biosensors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+cytof+system/helix+cyto+labeling+kit+red+dye+1/pmc11130809-106-9-17
Average 90 stars, based on 1 article reviews
helix cyto labeling kit red dye 1 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
helix cyto device  (Dynamic Biosensors)
90
Dynamic Biosensors helix cyto device
Real-time binding kinetics on EGFR and PD-L1 double-positive A431 and A549 cells. Real-time binding curves of ( A , B ) HCP-LCE and ( C , D ) LCE-E measured on ( A , C ) A431 and ( B , D ) A549 via real-time interaction cytometry (RT-IC) in a <t>heliX</t> <t>cyto</t> biosensor. The cells were fixed with PFA and loaded into the five individual cell traps on the chip. Fluorescently labeled analytes were injected in increasing concentrations in three subsequent injection steps. The association of the analytes was visibly reflected by an increase in the fluorescent signal. After the third injection, the system switches to buffer flow to monitor the dissociation of the analytes, which visibly manifests as a decrease in signal. Data points were fitted with a kinetic model that assumes monophasic association and biphasic dissociation.
Helix Cyto Device, supplied by Dynamic Biosensors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+cytof+system/helix++biosensor/pmc11130809-105-10-13
Average 90 stars, based on 1 article reviews
helix cyto device - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
mibi-tof mass spectrometers  (IonPath Inc)
90
IonPath Inc mibi-tof mass spectrometers
Real-time binding kinetics on EGFR and PD-L1 double-positive A431 and A549 cells. Real-time binding curves of ( A , B ) HCP-LCE and ( C , D ) LCE-E measured on ( A , C ) A431 and ( B , D ) A549 via real-time interaction cytometry (RT-IC) in a <t>heliX</t> <t>cyto</t> biosensor. The cells were fixed with PFA and loaded into the five individual cell traps on the chip. Fluorescently labeled analytes were injected in increasing concentrations in three subsequent injection steps. The association of the analytes was visibly reflected by an increase in the fluorescent signal. After the third injection, the system switches to buffer flow to monitor the dissociation of the analytes, which visibly manifests as a decrease in signal. Data points were fitted with a kinetic model that assumes monophasic association and biphasic dissociation.
Mibi Tof Mass Spectrometers, supplied by IonPath Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+cytof+system/mibi+tof+instrument/pm36240778-344-148-154
Average 90 stars, based on 1 article reviews
mibi-tof mass spectrometers - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
40-μm cell strainer  (pluriSelect Life Science UG Co KG)
90
pluriSelect Life Science UG Co KG 40-μm cell strainer
Real-time binding kinetics on EGFR and PD-L1 double-positive A431 and A549 cells. Real-time binding curves of ( A , B ) HCP-LCE and ( C , D ) LCE-E measured on ( A , C ) A431 and ( B , D ) A549 via real-time interaction cytometry (RT-IC) in a <t>heliX</t> <t>cyto</t> biosensor. The cells were fixed with PFA and loaded into the five individual cell traps on the chip. Fluorescently labeled analytes were injected in increasing concentrations in three subsequent injection steps. The association of the analytes was visibly reflected by an increase in the fluorescent signal. After the third injection, the system switches to buffer flow to monitor the dissociation of the analytes, which visibly manifests as a decrease in signal. Data points were fitted with a kinetic model that assumes monophasic association and biphasic dissociation.
40 μm Cell Strainer, supplied by pluriSelect Life Science UG Co KG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+cytof+system/40+%CE%BCm+cell+strainer/pm33950581-57-80-83
Average 90 stars, based on 1 article reviews
40-μm cell strainer - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
cytof system  (fluidigm)
95
fluidigm cytof system
Real-time binding kinetics on EGFR and PD-L1 double-positive A431 and A549 cells. Real-time binding curves of ( A , B ) HCP-LCE and ( C , D ) LCE-E measured on ( A , C ) A431 and ( B , D ) A549 via real-time interaction cytometry (RT-IC) in a <t>heliX</t> <t>cyto</t> biosensor. The cells were fixed with PFA and loaded into the five individual cell traps on the chip. Fluorescently labeled analytes were injected in increasing concentrations in three subsequent injection steps. The association of the analytes was visibly reflected by an increase in the fluorescent signal. After the third injection, the system switches to buffer flow to monitor the dissociation of the analytes, which visibly manifests as a decrease in signal. Data points were fitted with a kinetic model that assumes monophasic association and biphasic dissociation.
Cytof System, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+cytof+system/Maxpar+Water/pm35216676-261-7-9
Average 95 stars, based on 1 article reviews
cytof system - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
chromodomain helicase dna binding protein 5 chd5 cytosection  (OriGene)
N/A
Transient overexpression of CHD5 NM 015557 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 5 slides per pack
   Buy from Supplier
cathelicidin camp cytosection  (OriGene)
N/A
Transient overexpression of CAMP NM 004345 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 5 slides per pack
   Buy from Supplier
endothelin 2 edn2 cytosection  (OriGene)
N/A
Transient overexpression of EDN2 NM 001956 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 5 slides per pack
   Buy from Supplier
endothelin 1 edn1 cytosection  (OriGene)
N/A
Transient overexpression of EDN1 NM 001955 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 5 slides per pack
   Buy from Supplier
helicobacter pylori cytotoxicity-associated immunodominant antibody (fitc)  (biorbyt)
N/A
Rabbit polyclonal antibody against Helicobacter pylori Cytotoxicity-associated immunodominant conjugated to FITC Isotype Note: IgG Host Note: Rabbit Conjugation Note: FITC Reactivity Note: Bacterial Application Note: ELISA, WB
   Buy from Supplier
endothelin b receptor ednrb cytosection  (OriGene)
N/A
Transient overexpression of EDNRB NM 001122659 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 25 slides per pack
   Buy from Supplier

Image Search Results


Schematic of experimental design and data analyses. Plasma and sera from 27 individuals with Long COVID (LC) and 16 individuals without LC (Non-LC) were subjected to Olink and serological analyses. PBMCs from the same individuals were subjected to RNAseq analysis, as well as to CyTOF analysis at baseline, or following a 6-hour stimulation with peptides derived from SARS-CoV-2 spike proteins (see Methods) to analyze T cell responses. The cells for CyTOF were treated with viability marker, fixed, and stained with a 39-parameter panel prior to analysis on a CyTOF instrument. The indicated tools on the right were then used for analyses of the resulting high-dimensional datasets.

Journal: bioRxiv

Article Title: Long COVID manifests with T cell dysregulation, inflammation, and an uncoordinated adaptive immune response to SARS-CoV-2

doi: 10.1101/2023.02.09.527892

Figure Lengend Snippet: Schematic of experimental design and data analyses. Plasma and sera from 27 individuals with Long COVID (LC) and 16 individuals without LC (Non-LC) were subjected to Olink and serological analyses. PBMCs from the same individuals were subjected to RNAseq analysis, as well as to CyTOF analysis at baseline, or following a 6-hour stimulation with peptides derived from SARS-CoV-2 spike proteins (see Methods) to analyze T cell responses. The cells for CyTOF were treated with viability marker, fixed, and stained with a 39-parameter panel prior to analysis on a CyTOF instrument. The indicated tools on the right were then used for analyses of the resulting high-dimensional datasets.

Article Snippet: The PFA-fixed samples were washed twice with CAS buffer (Standard BioTools) and then spiked with 10% (v/v) EQ™ Four Element Calibration Beads (Standard BioTools) diluted in CAS buffer, before loading onto a Helios CyTOF instrument (UCSF Parnassus Flow Core).

Techniques: Derivative Assay, Marker, Staining

Real-time binding kinetics on EGFR and PD-L1 double-positive A431 and A549 cells. Real-time binding curves of ( A , B ) HCP-LCE and ( C , D ) LCE-E measured on ( A , C ) A431 and ( B , D ) A549 via real-time interaction cytometry (RT-IC) in a heliX cyto biosensor. The cells were fixed with PFA and loaded into the five individual cell traps on the chip. Fluorescently labeled analytes were injected in increasing concentrations in three subsequent injection steps. The association of the analytes was visibly reflected by an increase in the fluorescent signal. After the third injection, the system switches to buffer flow to monitor the dissociation of the analytes, which visibly manifests as a decrease in signal. Data points were fitted with a kinetic model that assumes monophasic association and biphasic dissociation.

Journal: Antibodies

Article Title: Balancing the Affinity and Tumor Cell Binding of a Two-in-One Antibody Simultaneously Targeting EGFR and PD-L1

doi: 10.3390/antib13020036

Figure Lengend Snippet: Real-time binding kinetics on EGFR and PD-L1 double-positive A431 and A549 cells. Real-time binding curves of ( A , B ) HCP-LCE and ( C , D ) LCE-E measured on ( A , C ) A431 and ( B , D ) A549 via real-time interaction cytometry (RT-IC) in a heliX cyto biosensor. The cells were fixed with PFA and loaded into the five individual cell traps on the chip. Fluorescently labeled analytes were injected in increasing concentrations in three subsequent injection steps. The association of the analytes was visibly reflected by an increase in the fluorescent signal. After the third injection, the system switches to buffer flow to monitor the dissociation of the analytes, which visibly manifests as a decrease in signal. Data points were fitted with a kinetic model that assumes monophasic association and biphasic dissociation.

Article Snippet: The real-time kinetics on the cells were measured in a heliX cyto device (Dynamic Biosensors) with chip type M5 (CY-M5-1, Dynamic Biosensors).

Techniques: Binding Assay, Cytometry, Labeling, Injection